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Comet assay in evaluating deoxyribonucleic acid damage after out-of-hospital cardiac arrest [Anatol J Cardiol]
Anatol J Cardiol. 2017; 18(1): 31-38 | DOI: 10.14744/AnatolJCardiol.2017.7578  

Comet assay in evaluating deoxyribonucleic acid damage after out-of-hospital cardiac arrest

Radka Hazuková1, Martina Rezácová2, Renata Köhlerová2, Tomáš Tomek3, Eva Cermáková4, Jaromír Kocí5, Miloslav Pleskot1
11st Department of Internal Medicine - Cardioangiology, University Hospital Hradec Králové, Faculty of Medicine in Hradec Králové, Charles University in Prague; Hradec Králové-Czech Republic
21st Department of Medical Biochemistry, University Hospital Hradec Králové, Faculty of Medicine in Hradec Králové, Charles University in Prague; Hradec Králové-Czech Republic
3Department of Internal Medicine Chrudim, Regional Hospital Pardubice, Faculty of Health Studies, University of Pardubice; Chrudim-Czech Republic
41st Department of Computer Technology Center, University Hospital Hradec Králové, Faculty of Medicine in Hradec Králové, Charles University in Prague; Hradec Králové-Czech Republic
5Department of Emerency Medicine, University Hospital Hradec Králové, Faculty of Medicine in Hradec Králové, Charles University in Prague; Hradec Králové-Czech Republic

Objective: This study aimed to investigate whether out-of-hospital cardiac arrest (OHCA) may induce severe DNA damage measured using comet assay in successfully resuscitated humans and to evaluate a short-term prognostic role.
Methods: In this prospective, controlled, blinded study (1/2013–1/2014), 41 patients (age, 63±14 years) successfully resuscitated from non-traumatic OHCA and 10 healthy controls (age, 53±17 years) were enrolled. DNA damage [double-strand breaks (DSBs) and single-strand breaks (SSBs)] was measured using comet assay in peripheral lymphocytes sampled at admission. Clinical data were recorded (according to Utstein style). A good short-term prognosis was defined as survival for 30 days.
Results: Among the patients, there were 71% (29/41) short-term survivors. After OHCA, DNA damage (DSBs and SSBs) was higher (11.0±7.6% and 0.79±2.41% in tail) among patients than among controls (1.96±1.63% and 0.02±0.03% in tail), and it was more apparent for DSBs (p<0.001 and p=0.085). There was no difference in the DNA damage between patients with cardiac and non-cardiac etiology, or between survivors and non survivors. Among Utstein style parameters, ventricular fibrillation, asystole, and early electrical defibrillation influenced DSBs; none of the factors influenced SSBs. Factors influencing survival were SSBs, ventricular fibrillation, length of cardiopulmonary resuscitation by professionals ≤15 min, cardiogenic shock, and postanoxic encephalopathy. In contrast to DSBs [area under the curve (AUC)=0.520], SSBs seem to have a potential in prognostication (AUC=0.639).
Conclusion: This study for the first time demonstrates revelation of DNA damage using comet assay in patients successfully resuscitated from OHCA. Whether DNA damage measured using comet assay may be a prognostic marker remains unknown, although our data may encourage some suggestions.

Keywords: Cardiac arrest, out-of-hospital, DNA damage, comet assay, cardiopulmonary resuscitation, survivors


Radka Hazuková, Martina Rezácová, Renata Köhlerová, Tomáš Tomek, Eva Cermáková, Jaromír Kocí, Miloslav Pleskot. Comet assay in evaluating deoxyribonucleic acid damage after out-of-hospital cardiac arrest. Anatol J Cardiol. 2017; 18(1): 31-38

Corresponding Author: Radka Hazuková, Czech Republic


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